Resources for the Lab

Free Loaner Equipment Program (Thermal Cycler)
 Contact mbahr@mbl.edu directly to coordinate.

 High schools conducting Lab 2: DNA Extraction and Lab 3: Polymerase Chain Reaction can borrow the following equipment from the MBL for the week that they will be running the labs:
  • BioRad MyCycler (Thermal Cycler)
  • Eppendorf Microcentrifuge and minicentrifuge
  • Fisher Vortex
  • 6 sets of P10, P100, and P1000 Pipettes
  • 6 Digital Blue QX5 Computer Microscopes for PC
Please plan in advance to coordinate scheduling and shipping. There are no costs associated with this program. Our objective is to integrate this cutting edge technology to your classroom. We imagine that students versed in PCR will appreciate high standards and be well-ahead of the curve when looking for volunteer research opportunities in college or summer lab experiences. Hopefully these opportunities will have the effect of influencing some into a major or career in the life sciences.



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DNA Sequencing Collaboration with the Marine Biological Laboratory
Contact us directly to coordinate.

 You can coordinate a free sequencing collaboration with the MBL. In this effort, you will mail aliquots of either Wolbachia PCR products or Qiagen DNA templates (only those species that were positive for Wolbachia and when the controls worked perfectly) to the lab. Please coordinate with us in advance of shipping the templates to the MBL. Within a couple of weeks, we will send you sequences back or upload them on a website for retrieval so that you and your students can use these sequences in the Lab 5 DNA Sequence Analysis (Bioinformatics), in place of the already provided sequence in that lab.


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Master List of Laboratory Materials


Download the 2011 master list of equipment and supplies needed to implement the Wolbachia laboratories.



Alternate Protocols


The Wolbachia Project protocols were designed to optimize student success in the classroom. In some instances, a less expensive or high throughput alternate protocol may be preferred.
  • Protocol for DNA Isolation using Edward's Buffer Download.
  • Protocol for Sigma-Aldrich Extract-and-Amp Download.
  • Fast Blue Protocol Download.
  • Protocol for DNA Isolation using Promega Wizard SV Genomic DNA Purification System Download.
  • DNA Extraction using the BioRad InstaGene Matrix Kit (#732-6030EDU)
    • Teacher Copy Download
    • Student Copy Download
    • InstaGene is also part of the BioRad GMO Investigator Kit (#166-2500EDU).
  • DNA Gels using Sodium Boric Acid Buffer Download.





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